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  Examples  
 
The list shows an excerpt of biological systems tested with RAMOS:
calculation of profiability :
Cultivation of the yeast Hansenula polymorpha under
oxygen- and substrate limitation

area normale
procedure
procedure with
RAMOS
savings
raw material 2250 mL 290mL 87%
optimisation time 80h 53h 34%
labor time 16.5h 4.1h 75%
list of publications:
  1. Anderlei T., Büchs J., Device for sterile online measurement of the oxygen transfer rate in shaking flasks, Biochem. Eng. J. 7(2), 157-162, 2001
  2. Stöckmann C., Losen M.; Dahlems U., Gellissen G., Büchs J., Definition of reproducible screening conditions case sample Hansenula polymorphia, Chem.-Ing.-Tech. 74(5), 701, 2002
  3. SilberbachM., MaierB., ZimmermannM., BüchsJ., Glucose oxidation by Gluconobacter oxydans: characterization in shaking flasks, scale-up and optimization of the pH profile, Appl. Microbiol. Biotechnol. 62(1), 92-98, 2003
  4. Raval K.N., Hellwig S., Prakash G., Ramos-Plasencia A., Srivastava A., BüchsJ., Necessity of a Two-Stage Process for the Production of Azadirachtin-Related Limonoids in Suspension Cultures of Azadirachta indica, J. Biosc. Bioengng. 96(1), 16-22, 2003
  5. Stöckmann Ch., Losen M., Dahlems U., Knocke Ch., Gellissen G., Büchs J., Effect of oxygen supply on passaging and screening of Hansenula polymorpha strains producing a recombinant protein in test tube cultures, FEMS Yeast Res. 4(2), 195-205, 2003
  6. Stöckmann Ch., Maier U., Anderlei T., Knocke Ch., Gellissen G., Büchs J., The Oxygen Transfer Rate as Key Parameter for the Characterisation of Hansenula polymorpha Screening Cultures, J. Ind. Microbiol. Biotechnol. 30, 613-622, 2003
  7. Maier U., Losen M., Büchs J., Advances in understanding and modeling the gas-liquid mass transfer in shaking flasks, Biochem. Eng. J. 17(3), 155-168, 2004
  8. Danielson P.D., Büchs J., Stöckmann Ch., Fogleman J.C., Maximizing cell densities in miniprep-scale cultures with H15 medium and improved oxygen, Biochem. Eng. J. 17(3), 175-180, 2004
  9. Anderlei T., Zang W., Büchs J., Online respiration activity measurement (OTR, CTR, RQ) in shake flasks, Biochem. Eng. J. 17(3), 187-194, 2004
  10. Lotter St., Büchs J. Utilization of power input measurements for optimisation of culture conditions in shaking flasks, Biochem. Eng. J. 17(3), 195-204, 2004
  11. Peter C.P., Lotter St., Maier U., Büchs J., Impact of out-of-phase conditions on screening results in shaking flask experiments, Biochem. Eng. J. 17(3), 205-216, 2004
  12. Losen M., Lingen B., Pohl M., BüchsJ., Effect of oxygen-limitation and medium composition on Escherichia coli in small-scale cultures, Biotechnol. Progress. (accepted)
 
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